Arboviruses are maintained in a natural cycle that requires blood-sucking arthropod and vertebrate hosts.Arboviruses are believed to persistently infect their arthropod host without overt pathology and cause acute infection with viremia in their vertebrate Hot Chocolate host.We have focused on elucidating how a specific arbovirus, Rift Valley fever (RVF) virus, causes cytopathic effect in cells derived from vertebrates and non-cytopathic infection in cells derived from arthropods.We demonstrate that the vertebrate virulence factor, NSs, LEDs is functional in arthropod cells but is expressed at significantly lower levels in infected arthropod versus infected vertebrate cells.